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random primer dna labeling kit  (TaKaRa)


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    Structured Review

    TaKaRa random primer dna labeling kit
    Random Primer Dna Labeling Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6514 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+labeling+kit/Random+Primer/10__1007_slash_s11627___026___10635___8-99-18-23
    Average 96 stars, based on 6514 article reviews
    random primer dna labeling kit - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: Drosophila PQBP1 Regulates Learning Acquisition at Projection Neurons in Aversive Olfactory Conditioning
    Article Snippet: A total of 250 ng of dPQBP1 cDNA fragment (∼0.7 kbp, full-length ORF) was labeled with [α- 32 P]dCTP (GE Healthcare Bioscience) using a DNA labeling kit (Takara) and hybridized, and then analyzed with a BAS2000 (Fuji Film) and sequentially exposed to Kodak x-ray films at −80°C. .. A total of 250 ng of dPQBP1 cDNA fragment (∼0.7 kbp, full-length ORF) was labeled with [α- 32 P]dCTP (GE Healthcare Bioscience) using a DNA labeling kit (Takara) and hybridized, and then analyzed with a BAS2000 (Fuji Film) and sequentially exposed to Kodak x-ray films at −80°C. ..

    Article Title: Molecular characterization of latent fertility restorer loci for Honglian cytoplasmic male sterility in Oryza species
    Article Snippet: In Oryza species, several distinct cytoplasmic male sterility (CMS) systems have been identified.. Honglian (HL) is one of the mainstream breeding CMS lines in large-scale commercial production in China and southern Asia.. In order to increase restorer diversity, we screened 130 Oryza accessions to find new restorer loci for HL-CMS.

    Article Title: Identification and characterization of a POU transcription factor in the cotton bollworm, Helicoverpa armigera
    Article Snippet: The PCR products were electrophoresed on a 1.2% agarose gel and transferred onto a Hybond N + Nylon membrane (Amersham). .. The Har-POU cDNA was labeled with [α- 32 P]-dCTP as a probe using a random primed DNA labeling kit (TaKaRa). ..

    Article Title: The Role of Zinc in Copper Homeostasis of Aspergillus fumigatus
    Article Snippet: .. P 32 radioisotope labeling was performed with a DNA Labeling Kit (Takara, Shiga, Japan, 6045). ..

    Article Title: Differentiation of Japanese green tea cultivars as revealed by RFLP analysis of phenylalanine ammonia-lyase DNA.
    Article Snippet: Japanese green tea cultivars and 463 local tea plants including mountainous tea, yama-cha, were analyzed to determine the process of differentiation of Japanese tea plants using phenylalanine ammonia lyase (PAL) as a DNA marker.. The main DNA fragments detected by RFLP analysis, which were named A, B and D, were inherited as multiple allelic genes at one locus.. Japanese tea cultivars were divided into five groups according to RFLPs: AA, AB, AD, BD and DD.

    DNA Labeling:

    Article Title: Drosophila PQBP1 Regulates Learning Acquisition at Projection Neurons in Aversive Olfactory Conditioning
    Article Snippet: A total of 250 ng of dPQBP1 cDNA fragment (∼0.7 kbp, full-length ORF) was labeled with [α- 32 P]dCTP (GE Healthcare Bioscience) using a DNA labeling kit (Takara) and hybridized, and then analyzed with a BAS2000 (Fuji Film) and sequentially exposed to Kodak x-ray films at −80°C. .. A total of 250 ng of dPQBP1 cDNA fragment (∼0.7 kbp, full-length ORF) was labeled with [α- 32 P]dCTP (GE Healthcare Bioscience) using a DNA labeling kit (Takara) and hybridized, and then analyzed with a BAS2000 (Fuji Film) and sequentially exposed to Kodak x-ray films at −80°C. ..

    Article Title: Precise and heritable gene targeting in rice using a sequential transformation strategy
    Article Snippet: .. DNA labeling kit , Takara , Cat# 6045. .. RNeasy Plant mini kit , Qiagen , Cat# 74904.

    Article Title: Molecular characterization of latent fertility restorer loci for Honglian cytoplasmic male sterility in Oryza species
    Article Snippet: In Oryza species, several distinct cytoplasmic male sterility (CMS) systems have been identified.. Honglian (HL) is one of the mainstream breeding CMS lines in large-scale commercial production in China and southern Asia.. In order to increase restorer diversity, we screened 130 Oryza accessions to find new restorer loci for HL-CMS.

    Article Title: Identification and characterization of a POU transcription factor in the cotton bollworm, Helicoverpa armigera
    Article Snippet: The PCR products were electrophoresed on a 1.2% agarose gel and transferred onto a Hybond N + Nylon membrane (Amersham). .. The Har-POU cDNA was labeled with [α- 32 P]-dCTP as a probe using a random primed DNA labeling kit (TaKaRa). ..

    Article Title: A thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator of MFAP4 mRNA in immature T-cell lines
    Article Snippet: .. Radiolabeled DNA probes were synthesized from cDNA fragments using a DNA labeling Kit (Takara) and hybridized in ExpressHybTM Hybridization Solution (Clontech) at 50°C overnight. ..

    Article Title: Heterologous expression of heat-resistant obscure (Hero) proteins enhances thermotolerance in plants
    Article Snippet: .. DNA labeling kit , Takara , Cat# 6045. .. RNeasy Plant mini kit , Qiagen , Cat# 74904.

    Article Title: The Role of Zinc in Copper Homeostasis of Aspergillus fumigatus
    Article Snippet: .. P 32 radioisotope labeling was performed with a DNA Labeling Kit (Takara, Shiga, Japan, 6045). ..

    Article Title: Differentiation of Japanese green tea cultivars as revealed by RFLP analysis of phenylalanine ammonia-lyase DNA.
    Article Snippet: Japanese green tea cultivars and 463 local tea plants including mountainous tea, yama-cha, were analyzed to determine the process of differentiation of Japanese tea plants using phenylalanine ammonia lyase (PAL) as a DNA marker.. The main DNA fragments detected by RFLP analysis, which were named A, B and D, were inherited as multiple allelic genes at one locus.. Japanese tea cultivars were divided into five groups according to RFLPs: AA, AB, AD, BD and DD.

    Polymerase Chain Reaction:

    Article Title: Molecular characterization of latent fertility restorer loci for Honglian cytoplasmic male sterility in Oryza species
    Article Snippet: In Oryza species, several distinct cytoplasmic male sterility (CMS) systems have been identified.. Honglian (HL) is one of the mainstream breeding CMS lines in large-scale commercial production in China and southern Asia.. In order to increase restorer diversity, we screened 130 Oryza accessions to find new restorer loci for HL-CMS.

    Random Primed:

    Article Title: Molecular characterization of latent fertility restorer loci for Honglian cytoplasmic male sterility in Oryza species
    Article Snippet: In Oryza species, several distinct cytoplasmic male sterility (CMS) systems have been identified.. Honglian (HL) is one of the mainstream breeding CMS lines in large-scale commercial production in China and southern Asia.. In order to increase restorer diversity, we screened 130 Oryza accessions to find new restorer loci for HL-CMS.

    Article Title: Identification and characterization of a POU transcription factor in the cotton bollworm, Helicoverpa armigera
    Article Snippet: The PCR products were electrophoresed on a 1.2% agarose gel and transferred onto a Hybond N + Nylon membrane (Amersham). .. The Har-POU cDNA was labeled with [α- 32 P]-dCTP as a probe using a random primed DNA labeling kit (TaKaRa). ..

    Western Blot:

    Article Title: Molecular characterization of latent fertility restorer loci for Honglian cytoplasmic male sterility in Oryza species
    Article Snippet: In Oryza species, several distinct cytoplasmic male sterility (CMS) systems have been identified.. Honglian (HL) is one of the mainstream breeding CMS lines in large-scale commercial production in China and southern Asia.. In order to increase restorer diversity, we screened 130 Oryza accessions to find new restorer loci for HL-CMS.

    Synthesized:

    Article Title: A thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator of MFAP4 mRNA in immature T-cell lines
    Article Snippet: .. Radiolabeled DNA probes were synthesized from cDNA fragments using a DNA labeling Kit (Takara) and hybridized in ExpressHybTM Hybridization Solution (Clontech) at 50°C overnight. ..

    Hybridization:

    Article Title: A thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator of MFAP4 mRNA in immature T-cell lines
    Article Snippet: .. Radiolabeled DNA probes were synthesized from cDNA fragments using a DNA labeling Kit (Takara) and hybridized in ExpressHybTM Hybridization Solution (Clontech) at 50°C overnight. ..



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    Image Search Results


    Graphical depiction illustrating regulation on the transcription of a c-di-GMP synthase encoding gene by the cAMP signaling system (Top) The S. sonnei CMCC51592 wild-type and crp mutant strains were incubated in LB medium at 37 °C until the OD 600 was 0.8. Then, 1.0 mL of the cultured bacterial suspension was transferred into a new 1.5 mL microcentrifuge tube. Total RNA was extracted, and cDNA was synthesized via reverse transcription. Primers were designed based on genes encoding key c-di-GMP metabolic enzymes, and RT–qPCR was performed to determine the effect of crp deletion on the expression of these genes. (Bottom) Biotin was used as a marker to label the purified promoter DNA fragment at its 3′ end to create a probe sample. DNA–protein (with or without cAMP) binding reactions were prepared by coincubating biotin-labelled probes with proteins. The DNA–protein complexes were separated from the unbound probes using a 5% polyacrylamide gel. Biotin-labelled probes with different mobilities were detected on the membrane. Figure reprinted with permission from Wang et al., 2025. Created with BioRender.

    Journal: STAR Protocols

    Article Title: Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

    doi: 10.1016/j.xpro.2026.104353

    Figure Lengend Snippet: Graphical depiction illustrating regulation on the transcription of a c-di-GMP synthase encoding gene by the cAMP signaling system (Top) The S. sonnei CMCC51592 wild-type and crp mutant strains were incubated in LB medium at 37 °C until the OD 600 was 0.8. Then, 1.0 mL of the cultured bacterial suspension was transferred into a new 1.5 mL microcentrifuge tube. Total RNA was extracted, and cDNA was synthesized via reverse transcription. Primers were designed based on genes encoding key c-di-GMP metabolic enzymes, and RT–qPCR was performed to determine the effect of crp deletion on the expression of these genes. (Bottom) Biotin was used as a marker to label the purified promoter DNA fragment at its 3′ end to create a probe sample. DNA–protein (with or without cAMP) binding reactions were prepared by coincubating biotin-labelled probes with proteins. The DNA–protein complexes were separated from the unbound probes using a 5% polyacrylamide gel. Biotin-labelled probes with different mobilities were detected on the membrane. Figure reprinted with permission from Wang et al., 2025. Created with BioRender.

    Article Snippet: Biotin 3′ End DNA Labeling Kit , KeyGEN BioTECH , Cat#KGS132.

    Techniques: Mutagenesis, Incubation, Cell Culture, Suspension, Synthesized, Reverse Transcription, Quantitative RT-PCR, Expressing, Marker, Purification, Binding Assay, Membrane